《植物生理学报》 2016, 52(12): 1891-1900

萌发期硫酸铜胁迫对绿豆生长的影响及其细胞内密度变化反应

傅涵¹, 石琴琴¹, 钟鸣², 刘誉^1,*

¹暨南大学医学院生化系, 广州510632, ²广州市东来生物科技有限公司, 广州510530

通信作者：刘誉；E-mail: xyuliu05@126.com

摘　要：

绿豆(Vigna radiata)在硫酸铜胁迫下水培发芽后再移植土种, 观察胚芽和幼苗生长情况; 测定胚芽和幼苗细胞内密度变化(DANCE反应), 以及胚芽的超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量, 研究萌发期硫酸铜胁迫对绿豆生长的影响及其DANCE反应。结果显示, 硫酸铜胁迫抑制胚芽生长, 以胁迫3~4 d最为显著, 并出现芽细胞DANCE反应。移植土壤后, 胁迫组幼苗出现代偿性加快生长, 生长率随萌发期硫酸铜胁迫浓度增加而提高。土种3 d后, 胁迫组幼苗表观生长状态恢复正常, 但是, 胁迫组幼苗根、茎、叶均出现DANCE反应。根、茎、叶部在土种1~2 d后均发生DANCE反应, 其中根部反应持续1 d, 茎部反应持续3 d, 而叶部反应则持续至土种第5日。土种6 d后, 各胁迫组幼苗均无DANCE反应。硫酸铜胁迫还导致胚芽SOD活性和MDA含量显著增高, SOD基因mRNA表达增加, 提示硫酸铜胁迫促进胚芽产生自由基。研究结果表明, 绿豆的DANCE反应可能是发芽减慢和幼苗代偿性生长所致, 发芽期适当的环境胁迫可能有助于植物幼苗生长。

关键词：绿豆; 硫酸铜; 胁迫; DANCE

收稿：2016-08-01   修定：2016-11-24

资助：中国科技部科技创新基金(12C26214405312)。

Effect of copper sulphate stress during germination on the growth of mung bean and the intracellular density change response

FU Han¹, SHI Qin-Qin¹, ZHONG Ming², LIU Yu^1,*

¹Department of Biochemistry, Medical College, Jinan University, Guangzhou 510632, China; ²Dawnlight Biotech Company, Guangzhou 510530, China

Corresponding author: LIU Yu; E-mail: xyuliu05@126.com

Abstract:

Mung beans were cultured in water for germination under stress of copper sulphate and then transplanted to soil, in order to study the effects of copper sulphate stress during germination on the growth of mung beans in soil and the intracellular density change (DANCE response). Mung bean germination in water and the seedling growth in soil were observed, and growth rates of the seedlings were calculated. Mung bean sprouts and seedlings were determined for DANCE response, and the sprouts were determined for the levels of superoxide dismutase (SOD) activity and malondialdehyde (MDA) contents. The results show that copper sulphate stress inhibited mung bean germination, significantly in day 3 and 4, with DANCE response in the cells. After transplantation to soil, all seedlings of the stressed groups showed compensatory growth, and the growth rates increased with the concentration of copper sulphate. In day 3 after transplantation to soil, the seedlings of all stressed groups apparently recovered normal growth. Seedlings of all stressed groups showed DANCE response in roots, stems and leaves in day 1 after transplantation, which lasted for 1 day in roots, 3 days in stems, and 5 days in leaves. However, in day 6 all seedlings showed no DANCE response. SOD activity and MDA content significantly increased in the sprouts after copper sulphate stress, with elevated levels of SOD mRNAs, indicating increased production of free radicals. The present study suggests that DANCE response in the mung beans might be a result of delayed germination in water and compensatory growth after transplantation; appropriate stress during germination might promote the growth of plantseedlings.

Key words: mung bean; copper sulphate; stress; DANCE